Biology 2401 - Microbiology (Every Fall)
Course Description
Prerequisites:
1) Principles of Biological Science (1401/1400) or Human Anatomy and
Physiology (1300 and 1301) and
2) Chemistry 1400 or Chemistry 1402
Textbook
Microbiology: Principles and Applications
By Jacquelyn Black 4th Edition (NEW)
http://cw.prenhall.com/black/
Laboratory Text
Microbiology: A Laboratory Manual
By Cappuccino/Sherman 5th Edition
Click here for website
Microbiology-Lecture Syllabus
Topic / Chapter
Scope and History of Microbiology 1
Fundamentals of Chemistry 2
Fundamentals of Chemistry II 2
Microscopy and Staining 3
Prokaryotic Cells and Eukaryotic Cells 4
Prokaryotic Cells and Eukaryotic Cells 4
Growth and Culture of Bacteria 6
Test #1
Metabolism 5
Metabolism II 5
Genetics I 7
Genetics II 7/8
Genetics III 8
Genetics IV 8
Test #2
Taxonomy / Bacteria 9/10
Viruses 11
Eukaryotic Microbes and Parasites 12
Eukaryotic Microbes and Parasites 12
Sterilization and Disinfection 13
Antimicrobial Therapy 14
Antimicrobial Therapy 14
Test#3
Host-Microbe Relationships and Disease Process 15
Epidemiology and Nosocomial Infection 16
Immunology I 17, 18, 19
Immunology II 17, 18, 19
Immunology III 17, 18, 19
Immunology IV 17, 18, 19
Diseases 20, 21, 22, 23
Final Exam
Lab Syllabus Microbiology 2401 - Microbiology laboratory
Check in, Microscope Ex. 4 and 5
Isolation of Microorganisms for Identification. (Unknown #1).Ex. 1,2,3
Media Preparation.
Receive Unknown # 2; Restreak Unknowns #1 and #2. Ex. 7 Simple stain
Ex. 3 Colonial Morphology. Transfer Unknowns # 1 and #2 to slants.
Ex. 10, Acid-Fast stain, Ex. 11 Endospore stain.
Ex. 9. Gram stain, Ex. 8. Negative stain.
Effect of Temperature on Growth, Motility Determination
Ex. 17 and 18. Effect of oxygen on growth.
Catch up day
Hydrolysis of Large Extracellular Molecules (Proteins, Lipids and Polysaccharides).
Ex. 14. Selective and Differential Media (PEA and EMB), Part V. Ex. 22 Utilization of Carbohydrates.
Ex. 13, Inoculation of Enriched Media. Ex. 30 Oxidase test, Ex. 29, Catalase test
Ex. 27 Litmus Milk Reactions
Ex. 24B and C: MR and VP Tests; Nitrate reduction.
1. Laboratory exam #1
Ex. 24A, Indole Production, Ex. 25 Hydrogen Sulfide Production (SIM Medium)
Ex. 24 D, Citrate utilization, Ex. 21 Gelatin hydrolysis
Wine making
Ex. 31, Identification of Unknowns, Bergey’s Manual
Ex. 40 Thermal Resistance of Microorganisms.
Part XI. Ex. 50 A,B,C and Ex. 51 Analysis of water
Ex. 47 Analysis of Food (Milk) Physical Agents of Control;
Ex. 41, Effect of NaCl on Microorganisms,
Ex. 42 Effect of Ultraviolet Light on Microorganisms.
Chemical Methods of Control;
Ex. 45, Disinfectants and Antiseptics,
Ex. 43A and B. Kirby-Bauer Antibiotic Sensitivity.
Phenotypic and Genotypic variation
Lecture Text Chapter 19;
ABO typing, Rh factor, Erythroblastosis.
Laboratory exam # 2
Interesting Link for Microbiology
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